Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Wnt1 Boosts Fracture Healing by Enhancing Bone Formation in the Fracture Callus.

doi: 10.1002/jbmr.4797

Figure Lengend Snippet: Fig. 7. Effects of recombinant Wnt1 on critical-size bone defect healing. (A) Experimental design. (B) Representative 3D reconstructions from μCT datasets. The entire area between the two inner pin holes in shown. Red indicates less mineralized bone, whereas yellow and blue indicate higher mineralized bone. (C) Bone volume in the defect area. (D) Polar moment of inertia of the bone in the defect area as analyzed by μCT evaluation. (E) Trabecular thickness, (F) trabecular separation, and (G) trabecular number of the newly formed bone in the defect area. (H) Active YAP1 and BMP2 immunostaining in the defect area. Scale bar = 100 μm. n = 7 per group. Mann-Whitney U test. sFRP1, secreted frizzled-related protein 1.

Article Snippet: The critical-size model has been described. (31) Five micrograms (5 μg) of recombinant sFRP1 protein alone (R&D Systems; 9019-SF-025) was used for the control group.

Techniques: Recombinant, Immunostaining, MANN-WHITNEY

Journal: Frontiers in oncology

Article Title: CAGE-miR-140-5p-Wnt1 Axis Regulates Autophagic Flux, Tumorigenic Potential of Mouse Colon Cancer Cells and Cellular Interactions Mediated by Exosomes.

doi: 10.3389/fonc.2019.01240

Figure Lengend Snippet: FIGURE 8 | CAGE and miR-140-5p regulate the expression of wnt1. (A) Potential binding of miR-140-5p to 3 ′-UTR of wnt1. (B) Wild type Luc-p62-3′-UTR or mutant Luc-p62-3′-UTR was transfected along with control mimic or miR-135-5p mimic (each at 10 nM) into the indicated cell line. At 48 h after transfection, luciferase activity assays were performed. *p < 0.05. (C) Cell lysates from the indicated cancer cells were subjected to qRT-PCR analysis. *p < 0.05. (D) Cell lysates from the indicated cancer cells were subjected to immunoblot. (E) The indicated cancer cells were transfected with the indicated siRNA (each at 10 nM) for 48 h, followed by immunoblot. (F) Same as E except that qRT-PCR analysis was performed. *p < 0.05; ***p < 0.0005. (G) The indicated cancer cells were transfected with control inhibitor (10 nM) for 48 h or miR-140-5p inhibitor (10 nM) for various time intervals. (H) The indicated cancer cells were transfected with control mimic (10 nM) for 48 h or miR-140-5p mimic (10 nM) for various time intervals, followed by immunoblot. (I) The indicated cancer cells were transfected with the indicated siRNA (each at 10 nM) for 48 h, followed by immunoblot analysis.

Article Snippet: Recombinant wnt1 protein was purchased from R&D systems.

Techniques: Expressing, Binding Assay, Mutagenesis, Transfection, Control, Luciferase, Activity Assay, Quantitative RT-PCR, Western Blot

Journal: Frontiers in oncology

Article Title: CAGE-miR-140-5p-Wnt1 Axis Regulates Autophagic Flux, Tumorigenic Potential of Mouse Colon Cancer Cells and Cellular Interactions Mediated by Exosomes.

doi: 10.3389/fonc.2019.01240

Figure Lengend Snippet: FIGURE 13 | MiR-140-5p and wnt1 regulate cellular interactions. (A) The indicated cancer cells were transfected with the indicated siRNA (each at 10 nM) for 48 h. Culture medium was added to CT26 cells, mast cells or lung macrophages for 24 h, followed by immunoblot. (B) The indicated cancer cells were transfected with the indicated mimic (each at 10 nM) for 48 h. Culture medium was added to CT26 cells or lung macrophages for 24 h, followed by immunoblot.

Article Snippet: Recombinant wnt1 protein was purchased from R&D systems.

Techniques: Transfection, Western Blot

Journal: Frontiers in oncology

Article Title: CAGE-miR-140-5p-Wnt1 Axis Regulates Autophagic Flux, Tumorigenic Potential of Mouse Colon Cancer Cells and Cellular Interactions Mediated by Exosomes.

doi: 10.3389/fonc.2019.01240

Figure Lengend Snippet: FIGURE 14 | Wnt1 mediates cellular interactions. (A) Recombinant wnt1 protein at the indicated concentration was added to CT26 cells for 24 h, followed by immunoblot. (B) CT26 cells were treated with recombinant wnt1 protein (10 ng/ml) for 24 h. Culture medium was then added to lung macrophages or lung mast cells for 24 h, followed by immunoblot. (C) Recombinant wnt1 protein (10 ng/ml) was added to macrophages for 24 h, followed by immunoblot. (D) Macrophages were treated with recombinant wnt1 protein (10 ng/ml) for 24 h. Culture medium was then added to CT26 cells for 24 h, followed by immunoblot.

Article Snippet: Recombinant wnt1 protein was purchased from R&D systems.

Techniques: Recombinant, Concentration Assay, Western Blot